In recent years, along with the people of the environmental problems of forest land, as the most important ecological system, its type of biomass research has become a hot issue of ecology. The study involved in global carbon forest carbon cycle of status for the greenhouse effect, the slow effect. As the forest ecosystem of the basic features of biomass, is thorough research problems and ecological problems many forestry key theoretical basis, the accurate determination of forest biomass in the world and ecologists historians. Based on the first-hand mastery of cold nature reserve area of forest resource continuous investigation data of forest fixed sample (2009), two kinds of investigation data according to the water main forest types of nature reserves, based on chebyshev orthogonal polynomial is constructing model, using biomass ChanMu biomass, VF supplement model programming languages, estimate the cool forest biomass of nature reserves, and analyzed. In recent years, along with the people of the environmental problems of forest land, as the most important ecological system, its type of biomass research has become a hot issue of ecology. The study involved in global carbon forest carbon cycle of status for the greenhouse effect, the slow effect. As the forest ecosystem of the basic features of biomass, is thorough research problems and ecological problems many forestry key theoretical basis, the accurate determination of forest biomass in the world and ecologists historians. Based on the first-hand mastery of cold nature reserve area of forest resource continuous investigation data of forest fixed sample (2009), two kinds of investigation data according to the water main forest types of nature reserves, based on chebyshev orthogonal polynomial is constructing model, using biomass ChanMu biomass, VF supplement model programming languages, estimate the cool forest biomass of nature reserves, and analyzed.