英语翻译帮我翻译下!本实验利用CTAB法提取了四角蛤蜊斧足的DNA,然后用琼脂糖凝胶对所提DNA进行电泳和紫外检测系统检测;选用提取较好的DNA样品作为PCR反应的模板,通过设计两条18SrRNA基因3′端引物,然后进行PCR扩增;PCR结束后,对所扩增的产物经琼脂糖凝胶电泳检测合格后送上海生工纯化,进行双向测序;所得序列用DNAStar软件组装,再进行校对.结果显示:四角蛤蜊18SrRNA基因3′端序列长度为629 bp,其A、T、G、C的含量分别为22.10%、26.55 %、24.80%、26.55
2019-06-19
英语翻译
帮我翻译下!
本实验利用CTAB法提取了四角蛤蜊斧足的DNA,然后用琼脂糖凝胶对所提DNA进行电泳和紫外检测系统检测;选用提取较好的DNA样品作为PCR反应的模板,通过设计两条18SrRNA基因3′端引物,然后进行PCR扩增;PCR结束后,对所扩增的产物经琼脂糖凝胶电泳检测合格后送上海生工纯化,进行双向测序;所得序列用DNAStar软件组装,再进行校对.结果显示:四角蛤蜊18SrRNA基因3′端序列长度为629 bp,其A、T、G、C的含量分别为22.10%、26.55 %、24.80%、26.55 %,G+C含量为51.35%;与别的同学所做的片段进行拼接,再进行比对分析其亲源关系.
优质解答
这样的 Experimental use of the CTAB extraction of 15.40 clams Hammer adequate DNA,Then on agarose gel electrophoresis of DNA detection systems and UV detection; Extraction better use DNA samples as PCR template,the reaction through the design of two 18S rRNA gene-3 'primers,and then PCR PCR after amplification on the expansion of the product by agarose gel electrophoresis detection qualified evacuation Shanghai Public Health purified - 377; Sequences from using DNA Star software assembly and then proofread.The results showed :15.40 clams 18S rRNA gene 3 'sequence length of 629 bp,A,T,G,C content of 22.10%,26.55 %,24.80%,26.55% G + C content of 51.35%; with other students doing the fragments were spliced further than its analysis of the pro-source relationship.
这样的 Experimental use of the CTAB extraction of 15.40 clams Hammer adequate DNA,Then on agarose gel electrophoresis of DNA detection systems and UV detection; Extraction better use DNA samples as PCR template,the reaction through the design of two 18S rRNA gene-3 'primers,and then PCR PCR after amplification on the expansion of the product by agarose gel electrophoresis detection qualified evacuation Shanghai Public Health purified - 377; Sequences from using DNA Star software assembly and then proofread.The results showed :15.40 clams 18S rRNA gene 3 'sequence length of 629 bp,A,T,G,C content of 22.10%,26.55 %,24.80%,26.55% G + C content of 51.35%; with other students doing the fragments were spliced further than its analysis of the pro-source relationship.