求翻译一段分子生物学方面的论文摘要,不要软件直接翻译的 要专业点的 谢了对DNA结合蛋白的研究可以了解基因信息的复制、调节和表达调控等分子基础,进而揭示细胞发育、分化、死亡等生命的基本活动规律.凝胶电泳是分析复杂蛋白质混合物、分析某一蛋白质或者多肽相对含量的强有力的生化分析方法.此次实验采用1.4mol/L NaCl溶液提取DNP,再采用10倍稀释法沉淀DNP,裂解DNP后采用SDS-聚丙烯酰胺凝胶电泳分析,再采用几种适用于DNA结合蛋白电泳的考马斯亮蓝染色方法染色,实验证明,考马斯亮蓝R-250的染色效
2019-03-30
求翻译一段分子生物学方面的论文摘要,不要软件直接翻译的 要专业点的 谢了
对DNA结合蛋白的研究可以了解基因信息的复制、调节和表达调控等分子基础,进而揭示细胞发育、分化、死亡等生命的基本活动规律.凝胶电泳是分析复杂蛋白质混合物、分析某一蛋白质或者多肽相对含量的强有力的生化分析方法.此次实验采用1.4mol/L NaCl溶液提取DNP,再采用10倍稀释法沉淀DNP,裂解DNP后采用SDS-聚丙烯酰胺凝胶电泳分析,再采用几种适用于DNA结合蛋白电泳的考马斯亮蓝染色方法染色,实验证明,考马斯亮蓝R-250的染色效果优于考马斯亮蓝G-250.
不要软件翻译的.谢了额
要翻译成英文.
优质解答
怎么又有一段了?虽然我给你翻,但是对内容却不同意.研究DNA结合蛋白要用gel shift,而不是染色.你的研究思路不对.
好了,翻译如下：
By studying DNA binding protein, we can get the fundamental molecular knowledge of the replication, regulation and control of expression of genetic information. This will help to reveal the mechanism of some basic cellular process such as development, differentiation and death. The current experiment utilized 1.4M NaCl solution to extract nuclear protein (DNP), followed by 10 times dilution protocol to precipitate DNP. After lysis of DNP, SDS-PAGE was used to analyze the protein. Several Coomassie stain protocols that for DNA binding protein were used. Our results showed that Coomassie R-250 was better than G-250 in term of stain effect.
怎么又有一段了?虽然我给你翻,但是对内容却不同意.研究DNA结合蛋白要用gel shift,而不是染色.你的研究思路不对.
好了,翻译如下：
By studying DNA binding protein, we can get the fundamental molecular knowledge of the replication, regulation and control of expression of genetic information. This will help to reveal the mechanism of some basic cellular process such as development, differentiation and death. The current experiment utilized 1.4M NaCl solution to extract nuclear protein (DNP), followed by 10 times dilution protocol to precipitate DNP. After lysis of DNP, SDS-PAGE was used to analyze the protein. Several Coomassie stain protocols that for DNA binding protein were used. Our results showed that Coomassie R-250 was better than G-250 in term of stain effect.